| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Clostridium ljungdahlii | TGCGGTAAACCAAGCAAAGC | TCTTGCTGGGACACCTTCAG | 59.97 | 59.60 | 294 | 126 |
100.00%
|
100.00%
|
| Primer 62 | Clostridium ljungdahlii | ACATCAACCGGAGTACTGGA | AGTGCTCTAGCCAATGCCA | 58.07 | 59.01 | 185 | 125 |
100.00%
|
100.00%
|
| Primer 63 | Clostridium ljungdahlii | ACCATAGTAGGCGATGCTGT | AACTCCAGCTCCTGACCCA | 58.59 | 60.15 | 162 | 125 |
100.00%
|
100.00%
|
| Primer 64 | Clostridium ljungdahlii | ACCATAGTAGGCGATGCTGT | TAACTCCAGCTCCTGACCCA | 58.59 | 59.88 | 163 | 125 |
100.00%
|
100.00%
|
| Primer 65 | Clostridium ljungdahlii | TCTAAGGGGAAGCGGATGC | TCTAGTTCACTGGCACTTCCA | 59.17 | 58.67 | 171 | 125 |
100.00%
|
100.00%
|
| Primer 66 | Clostridium ljungdahlii | GCGGATGCTTTTGGGGAAA | TCTAGTTCACTGGCACTTCCA | 59.03 | 58.67 | 160 | 125 |
100.00%
|
100.00%
|
| Primer 67 | Clostridium ljungdahlii | TTCTAAGGGGAAGCGGATGC | TCTAGTTCACTGGCACTTCCA | 59.82 | 58.67 | 172 | 125 |
100.00%
|
100.00%
|
| Primer 68 | Clostridium ljungdahlii | GCGGATGCTTTTGGGGAAAC | TCTAGTTCACTGGCACTTCCA | 60.39 | 58.67 | 160 | 125 |
100.00%
|
100.00%
|
| Primer 69 | Clostridium ljungdahlii | AGTGGCTGCAGCTTCCAT | ACTGCCGTTAATGCCTCCA | 59.24 | 59.32 | 273 | 125 |
100.00%
|
100.00%
|
| Primer 70 | Clostridium ljungdahlii | ACTTCCAGGCTGTCCATGC | AGGCCAGTTCACGTACCCA | 60.00 | 60.84 | 274 | 125 |
100.00%
|
100.00%
|